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WB analysis using 66207-1-Ig (same clone as 66207-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 66207-1-Ig (same clone as 66207-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 66207-1-Ig (B2M Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66207-1-Ig (same clone as 66207-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66207-1-Ig (same clone as 66207-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.
IF Staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Beta-2-Microglobulin antibody (66207-1-Ig, Clone: 1C3B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

Beta-2-Microglobulin Monoclonal antibody, PBS Only

Beta-2-Microglobulin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66207-1-PBS

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

B2M, 1C3B7, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66207-1-PBS targets Beta-2-Microglobulin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	beta-2-microglobulin
Calculated Molecular Weight	14 kDa
Observed Molecular Weight	13 kDa
GenBank Accession Number	BC032589
Gene Symbol	B2M
Gene ID (NCBI)	567
ENSEMBL Gene ID	ENSG00000166710
RRID	AB_2881598
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P61769
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.