Beta-2-Microglobulin Monoclonal antibody, PBS Only

Beta-2-Microglobulin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C3B7

Cat no : 66207-1-PBS

Synonyms

B2M, beta 2 microglobulin, Beta-2-microglobulin, HDCMA22P

---

Validation Data Gallery

• 

  WB analysis using 66207-1-Ig (same clone as 66207-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66207-1-Ig (same clone as 66207-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66207-1-Ig (B2M Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66207-1-Ig (Beta-2-Microglobulin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 66207-1-Ig (same clone as 66207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 66207-1-Ig (same clone as 66207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66207-1-Ig (B2M Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 66207-1-Ig (same clone as 66207-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Beta-2-Microglobulin antibody (66207-1-Ig, Clone: 1C3B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66207-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66207-1-PBS targets Beta-2-Microglobulin in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.