Bcl-XL Recombinant antibody, PBS Only (Capture)

Bcl-XL Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240450A9

Cat no : 83330-2-PBS

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

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Validation Data Gallery

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  IF Staining of HeLa using 83330-2-RR (same clone as 83330-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-xL antibody (83330-2-RR, Clone: 240450A9 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83330-2-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 83330-2-RR (same clone as 83330-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Bcl-xL Recombinant antibody (83330-2-RR, Clone:240450A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83330-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83330-2-RR (same clone as 83330-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-xL Recombinant antibody (83330-2-RR, Clone:240450A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83330-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00460-1

  Cytometric bead array standard curve of MP00460-1, Bcl-xL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83330-2-PBS. Detection antibody: 83330-3-PBS. Standard: Ag25604. Range: 0.625-80 ng/mL.

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Product Information

83330-2-PBS targets Bcl-XL as part of a matched antibody pair:

MP00460-1: 83330-2-PBS capture and 83330-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Bcl-XL fusion protein Ag25604
• Full Name: BCL2-like 1
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: BC019307
• Gene Symbol: Bcl-XL
• Gene ID (NCBI): 598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.