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ATG14/Barkor (C-terminal) Polyclonal antibody

ATG14/Barkor (C-terminal) Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 24412-1-AP


ATG14, Atg14L, Barkor, KIAA0831

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24412-1-AP is ATG14/Barkor (C-terminal) Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG14/Barkor (C-terminal) fusion protein Ag19707
Full Name KIAA0831
Calculated Molecular Weight 492 aa, 55 kDa
Observed Molecular Weight 65-68 kDa, 42 kDa
GenBank Accession NumberBC109090
Gene Symbol KIAA0831
Gene ID (NCBI) 22863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Barkor, also named as ATG14, Atg14L and KIAA0831, is named as Beclin 1-associated autophagy-related key regulator protein. The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation, and Salmonella typhimurium amplification. A major band around 65-68 kDa and a light band around 42 kDa can be observed in western blot which may be caused by alternative splicing of ATG14. The specificity of ATG14 antibody has been tested by siRNA test.


Product Specific Protocols
WB protocol for ATG14/Barkor (C-terminal) antibody 24412-1-APDownload protocol
IHC protocol for ATG14/Barkor (C-terminal) antibody 24412-1-APDownload protocol
IF protocol for ATG14/Barkor (C-terminal) antibody 24412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Differ

ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration.

Authors - Isaac Tamargo-Gómez

J Cell Sci

Syntaxin 17, an ancient SNARE paralog, plays different and conserved roles in different organisms.

Authors - Shun Kato

Microb Pathog

Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells.

Authors - Qiang Fu

Exp Eye Res

Inhibiting miR-129-5p alleviates inflammation and modulates autophagy by targeting ATG14 in fungal keratitis.

Authors - Jingqiong Lin


An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration

Authors - Peigang Liang