ATG14/Barkor (C-terminal) Polyclonal antibody

• Featured Product
• KD/KO Validated

ATG14/Barkor (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 24412-1-AP

Synonyms

Barkor, ATG14, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, Atg14L

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Validation Data Gallery

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  WB analysis of HeLa using 24412-1-AP

  WB result of ATG14/Barkor (C-terminal) antibody (24412-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG14/Barkor (C-terminal) transfected HeLa cells.

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  WB analysis of mouse brain using 24412-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 24412-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 24412-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 24412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 24412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 24412-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATG14/Barkor (C-terminal) antibody (24412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells
• Positive IHC detected in: human brain tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 2 publications below

Product Information

24412-1-AP targets ATG14/Barkor (C-terminal) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG14/Barkor (C-terminal) fusion protein Ag19707
• Full Name: KIAA0831
• Calculated Molecular Weight: 492 aa, 55 kDa
• Observed Molecular Weight: 65-68 kDa, 42 kDa
• GenBank Accession Number: BC109090
• Gene Symbol: ATG14
• Gene ID (NCBI): 22863
• RRID: AB_2879531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Barkor, also named as ATG14, Atg14L and KIAA0831, is named as Beclin 1-associated autophagy-related key regulator protein. The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation, and Salmonella typhimurium amplification. A major band around 65-68 kDa and a light band around 42 kDa can be observed in western blot which may be caused by alternative splicing of ATG14. The specificity of ATG14 antibody has been tested by siRNA test.

Publications

Species	Application	Title
mouse	WB	Cell Death Differ; ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration.; Authors - Isaac Tamargo-Gómez
human	IF	J Cell Sci; Syntaxin 17, an ancient SNARE paralog, plays different and conserved roles in different organisms.; Authors - Shun Kato
human	WB	Microb Pathog; Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells.; Authors - Qiang Fu
mouse	WB,IF	Exp Eye Res; Inhibiting miR-129-5p alleviates inflammation and modulates autophagy by targeting ATG14 in fungal keratitis.; Authors - Jingqiong Lin
human	WB	EMBO Rep; An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration; Authors - Peigang Liang