BTF3L3-Specific Polyclonal antibody

BTF3L3-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19753-1-AP

Synonyms

BTF3L3, HUMBTFD

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Validation Data Gallery

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  WB analysis of human brain using 19753-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19753-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 19753-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 19753-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 19753-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 19753-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19753-1-AP (BTF3L3-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human cervical cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19753-1-AP targets BTF3L3-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: basic transcription factor 3, like 3
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: Q13892
• Gene Symbol: BTF3L3
• Gene ID (NCBI): 132556
• RRID: AB_10637858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTF3L3 belongs to the NAC-beta family. It is a general transcription factor. It is required for the initiation of transcription. The antibody has no cross reaction to BTF3.