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BST2 Recombinant antibody, PBS Only

BST2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241513A1

Cat no : 84190-7-PBS

Synonyms

CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BST2 Recombinant antibody (84190-7-RR)

    WB analysis using 84190-7-RR (same clone as 84190-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84190-7-RR (BST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84190-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using BST2 Recombinant antibody (84190-7-RR)

    IHC staining of mouse liver using 84190-7-RR (same clone as 84190-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84190-7-RR (BST2 antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84190-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using BST2 Recombinant antibody (84190-7-RR)

    IHC staining of mouse liver using 84190-7-RR (same clone as 84190-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84190-7-RR (BST2 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84190-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BST2 Recombinant antibody (84190-7-RR)

    IF Staining of HeLa using 84190-7-RR (same clone as 84190-7-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BST2 antibody (84190-7-RR, Clone: 241513A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84190-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BST2 Recombinant antibody (84190-7-RR)

    IF Staining of HepG2 using 84190-7-RR (same clone as 84190-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BST2 antibody (84190-7-RR, Clone: 241513A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84190-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84190-7-RR

    Affinity and Kinetic Characterization of 84190-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84190-7-RR against Human BST2 were performed. The affinity constant is below 1 pM.

Product Information

84190-7-PBS targets BST2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein
Full Name bone marrow stromal cell antigen 2
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 30-36 kDa, 50-70 kDa
GenBank Accession NumberBC033873
Gene Symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).