BST2 Monoclonal antibody

• Featured Product
• KD/KO Validated

BST2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3C4B5

Cat no : 66919-1-Ig

Synonyms

CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2

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Validation Data Gallery

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  WB analysis using 66919-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66919-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66919-1-Ig

  WB result of BST2 antibody (66919-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BST2 transfected HeLa cells.

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  WB analysis of HepG2 using 66919-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66919-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood leukocyte using 66919-1-Ig

  Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66919-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66919-1-Ig (BST2 antibody) at dilution of 1:500 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human liver using 66919-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66919-1-Ig (BST2 antibody) at dilution of 1:500 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HeLa using 66919-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BST2 antibody (66919-1-Ig, Clone: 3C4B5 ) at dilution of 1:2500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human peripheral blood leukocyte, L02 cells, Jurkat cells, THP-1 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1250-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66919-1-Ig targets BST2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BST2 fusion protein Ag4430
• Full Name: bone marrow stromal cell antigen 2
• Calculated Molecular Weight: 180 aa, 20 kDa
• Observed Molecular Weight: 18 kDa, 30-36 kDa
• GenBank Accession Number: BC033873
• Gene Symbol: BST2
• Gene ID (NCBI): 684
• RRID: AB_2882246
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

Publications

Species	Application	Title
human		Mol Biol Cell; ER-to-Golgi trafficking of procollagen III via conventional vesicular and tubular carriers.; Authors - Yukihiro Hirata