BST2 Polyclonal antibody

• Featured Product
• KD/KO Validated

BST2 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30118-1-AP

Synonyms

Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin

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Validation Data Gallery

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  WB analysis of HeLa using 30118-1-AP

  WB result of BST2 antibody (30118-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BST2 transfected HeLa cells.

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  WB analysis of HeLa using 30118-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 30118-1-AP (BST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse eye using 30118-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using BST2 antibody (30118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 30118-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using BST2 antibody (30118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30118-1-AP targets BST2 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BST2 fusion protein Ag32333
• Full Name: bone marrow stromal cell antigen 2
• Calculated Molecular Weight: 180 aa, 20 kDa
• Observed Molecular Weight: 30-36 kDa, 50-70 kDa
• GenBank Accession Number: BC033873
• Gene Symbol: BST2
• Gene ID (NCBI): 684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).