BSEP Monoclonal antibody, PBS Only

BSEP Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C11D5

Cat no : 67512-1-PBS

Synonyms

ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP

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Validation Data Gallery

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  WB analysis using 67512-1-Ig (same clone as 67512-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67512-1-Ig (same clone as 67512-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67512-1-Ig (same clone as 67512-1-PBS)

  Unboiled HepG2 cell lysates, unboiled A549 cell lysates, unboilled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig lung using 67512-1-Ig (same clone as 67512-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67512-1-Ig (same clone as 67512-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 67512-1-Ig (same clone as 67512-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BSEP antibody (67512-1-Ig, Clone: 3C11D5 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67512-1-PBS targets BSEP in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BSEP fusion protein Ag29135
• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 11
• Calculated Molecular Weight: 146 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: NM_003742
• Gene Symbol: ABCB11
• Gene ID (NCBI): 8647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage. The calculated MW of ABCB11 is 146 kDa, 67512-1-Ig can detect bands around 150 kDa. It's reported that ABCB11 has several N-glycosylation sites, the observed higher molecular mass may due to posttranslational N-glycosylation. (PMID: 9545351, 24359682)