BRUNOL6 Polyclonal antibody

BRUNOL6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19078-1-AP

Synonyms

CUG-BP- and ETR-3-like factor 6, CELF-6, CELF6, CELF 6, Bruno-like protein 6

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Validation Data Gallery

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  WB analysis using 19078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19078-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 19078-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 19078-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 19078-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, rat brain tissue, pig brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

19078-1-AP targets BRUNOL6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRUNOL6 fusion protein Ag4970
• Full Name: bruno-like 6, RNA binding protein (Drosophila)
• Calculated Molecular Weight: 481 aa, 50 kDa
• Observed Molecular Weight: 50 kDa, 39 kDa
• GenBank Accession Number: BC030835
• Gene Symbol: BRUNOL6
• Gene ID (NCBI): 60677
• RRID: AB_2243662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Cell Death Dis; Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis; Authors - Bowen Liu