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  • Featured Product
  • KD/KO Validated

BRIP1(N-terminal) Polyclonal antibody

BRIP1(N-terminal) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24436-1-AP

Synonyms

BACH1, BRIP1, BRIP1(N-terminal), FANCJ, OF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRIP1(N-terminal) Polyclonal antibody (24436-1-AP)

    WB analysis using 24436-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24436-1-AP (BRIP1(N-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BRIP1(N-terminal) Polyclonal antibody (24436-1-AP)

    WB analysis of HeLa using 24436-1-AP

    WB result of BRIP1(N-terminal) antibody (24436-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRIP1(N-terminal) transfected HeLa cells.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24436-1-AP targets BRIP1(N-terminal) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRIP1(N-terminal) fusion protein Ag19907
Full Name BRCA1 interacting protein C-terminal helicase 1
Calculated Molecular Weight 1249 aa, 141 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC101472
Gene Symbol BACH1/BRIP1
Gene ID (NCBI) 83990
RRIDAB_2879548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRIP1 also named as BACH1, FANCJ or ATP dependent RNA helicase BRIP1 is a 1249 amino acid protein, which contains one helicase ATP-binding domain and belongs to the DEAD box heilicase family, DEAH subfamily. BRIP1 contains nuclear localization signal and is ubiquitously expressed with highest levels in testis, an expression pattern similar to that of BRCA1. BRIP1 is required to resolve the secondary structures of guanine-rich DNA that occasionally form during lagging-strand DNA synthesis.

Protocols

Product Specific Protocols
WB protocol for BRIP1(N-terminal) antibody 24436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Authors - Jiao Zhao
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Adrianna (Verified Customer) (10-31-2022)

This antibody is not as good as some of the other Proteintech antibodies (it has more background) and it looks like I am detecting two bands, however I can clearly see the correct band at around 140 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF7