BRF2 Polyclonal antibody, PBS Only

• 

  WB analysis of HT-1080 using 12056-1-AP (same clone as 12056-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12056-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 12056-1-AP (same clone as 12056-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12056-1-PBS in a different storage buffer formulation.

BRF2 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 12056-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

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Product Information

12056-1-PBS targets BRF2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRF2 fusion protein Ag2686
• Full Name: BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC010648
• Gene Symbol: BRF2
• Gene ID (NCBI): 55290
• RRID: AB_2218683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HAW0
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Human cells contain two types of RNA polymerase III transcription factor(TFIIIB), BRF1 and BRF2. BRF2,also named as BRFU, contains one TFIIB-type zinc finger. It is a general activator of RNA polymerase III transcription. It is a factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. BRF2 is recruited to type 3 promoters such as the human U6 snRNA promoter. It differs from BRF1-TFIIIB in that it contains the TFIIB-related factor BRF2 iinstead of BRF1 and its three components do not form a stable complex. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human BRF2. It recognizes a 50-60kd band in WB analysis.