BRE Polyclonal antibody

BRE Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11702-1-AP

Synonyms

BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2

---

Validation Data Gallery

• 

  WB analysis using 11702-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11702-1-AP (BRE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11702-1-AP

  IP result of anti-BRE (IP:11702-1-AP, 4ug; Detection:11702-1-AP 1:1000) with HeLa cells lysate 2320 ug.

• 

  IHC staining of human gliomas using 11702-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11702-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

11702-1-AP targets BRE in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRE fusion protein Ag2256
• Full Name: brain and reproductive organ-expressed (TNFRSF1A modulator)
• Calculated Molecular Weight: 383 aa, 44 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: BC001251
• Gene Symbol: BRE
• Gene ID (NCBI): 9577
• RRID: AB_2066049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Genes Dev; NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control.; Authors - Wang Bin B
human	WB	Int J Biol Sci; PPDPF Promotes the Progression and acts as an Antiapoptotic Protein in Non-Small Cell Lung Cancer.; Authors - Mu Yun