• Featured Product
  • KD/KO Validated

BRD8 Monoclonal antibody

BRD8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5B6D6

Cat no : 60121-1-Ig

Synonyms

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120



Tested Applications

Positive WB detected infetal human brain tissue, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60121-1-Ig targets BRD8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BRD8 fusion protein Ag0790
Full Name bromodomain containing 8
Calculated Molecular Weight 120 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC008039
Gene Symbol BRD8
Gene ID (NCBI) 10902
RRIDAB_2066025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].

Protocols

Product Specific Protocols
WB protocol for BRD8 antibody 60121-1-IgDownload protocol
IHC protocol for BRD8 antibody 60121-1-IgDownload protocol
IF protocol for BRD8 antibody 60121-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols