BRD4 Monoclonal antibody

BRD4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (2)

Applications

WB, ChIP, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2F7E2

Cat no : 67374-2-Ig

Synonyms

BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1

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Validation Data Gallery

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  WB analysis using 67374-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67374-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, HepG2 cells, LNcaP cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• ChIP: See 1 publications below

Product Information

67374-2-Ig targets BRD4 in WB, ChIP, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BRD4 fusion protein Ag27545
• Full Name: bromodomain containing 4
• Calculated Molecular Weight: 1362 aa, 152 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC035266
• Gene Symbol: BRD4
• Gene ID (NCBI): 23476
• RRID: AB_2918488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Bioengineered; Pathological stage-associated non-coding RNA long intergenic non-protein coding RNA 1234 (LINC01234) participation in cell cycle regulation in adrenocortical carcinoma through bromodomain-containing protein 4 (BRD4) expression mediation via sponging microRNA (miR)-140-3p.; Authors - Dan-Dan Zhu
human	WB,ChIP	Curr Med Sci; ARID1A Inactivation Increases Expression of circ0008399 and Promotes Cisplatin Resistance in Bladder Cancer; Authors - Yang-Kai Jiang
human		J Transl Med; Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation; Authors - Qinghui Kan