BRCA1 Monoclonal antibody

BRCA1 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

20D7G6

Cat no : 66267-1-Ig

Synonyms

BRCA1, BRCAI, BRCC1, breast cancer 1, early onset, BROVCA1, IRIS, PSCP, RING finger protein 53, RNF53

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Validation Data Gallery

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  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using (BRCA1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using (BRCA1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Published Applications

• IF: See 1 publications below

Product Information

66267-1-Ig targets BRCA1 in IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BRCA1 fusion protein Ag19178
• Full Name: breast cancer 1, early onset
• Calculated Molecular Weight: 1863 aa, 208 kDa
• Observed Molecular Weight: 207 kDa
• GenBank Accession Number: BC115037
• Gene Symbol: BRCA1
• Gene ID (NCBI): 672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RCA1, also named as RNF53, plays a central role in DNA repair by facilitating cellular response to DNA repair. It is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. It is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is required for FANCD2 targeting to sites of DNA damage. It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. The antibody is specific to BRCA1. BRCA1 appears to produce multiple splice variants. BRCA1 is a nuclear protein with a molecular mass of 220 kDa. The present study describes the isolation and expression of two cDNAs of BRCA1, including a splice variant designated BRCA1D672-4095. BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein. In contrast to BRCA1, BRCA1D672-4095 localizes to the cytoplasm.

Publications

Species	Application	Title
human	IF	Oncogene; RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.; Authors - Yang Yu