BRAF Polyclonal antibody

BRAF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 20899-1-AP

Synonyms

RAFB1, Proto-oncogene B-Raf, p94, EC:2.7.11.1, BRAF1

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Validation Data Gallery

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  WB analysis using 20899-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20899-1-AP (BRAF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20899-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20899-1-AP (BRAF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human malignant melanoma using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20899-1-AP (BRAF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20899-1-AP (BRAF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of NIH/3T3 using 20899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 20899-1-AP (BRAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 20899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BRAF antibody (20899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-29 cells, HEK-293 cells, HeLa cells, K-562 cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: human malignant melanoma tissue, human testis tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 1 publications below

Product Information

20899-1-AP targets BRAF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRAF fusion protein Ag15014
• Full Name: v-raf murine sarcoma viral oncogene homolog B1
• Calculated Molecular Weight: 766 aa, 84 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC101757
• Gene Symbol: BRAF
• Gene ID (NCBI): 673
• RRID: AB_2878760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B-Raf proto-oncogene serine/threonine kinase(BRAF), is a protein belonging to the raf/mil family of serine/threonine protein kinases. BRAF plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. BRAF is associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. BRAF is also associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. BRAF plays a role in the PD-1/PDL-1 pathway. In some cancers, BRAF is activated by rearrangements that fuse its kinase domain to 5' partner genes and then has different molecular weights (PMID: 23890088). BRAF has several isoforms, the calculated molecular weight of BRAF is 84 kDa, but the observed molecular weight is about 65 kDa(isoform).

Publications

Species	Application	Title
mouse	WB	Acta Pharmacol Sin; 1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia; Authors - Xiao-Wei Li
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
human	WB	Front Immunol; Immune Dysfunction Mediated by the ceRNA Regulatory Network in Human Placenta Tissue of Intrahepatic Cholestasis Pregnancy.; Authors - Yuya Wang
human	WB	Life Sci; Network pharmacology and molecular docking reveal the mechanism of Scopoletin against non-small cell lung cancer.; Authors - Chong Yuan
chicken	WB	Gene; WT1 suppresses follicle-stimulating hormone-induced progesterone secretion by regulating ERK1/2 pathway in chicken preovulatory granulosa cells.; Authors - Shuixin Tang