BPGM Polyclonal antibody

BPGM Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17173-1-AP

Synonyms

2,3 bisphosphoglycerate mutase, Bisphosphoglycerate mutase, BPG dependent PGAM, BPGM

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Validation Data Gallery

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  WB analysis of mouse liver using 17173-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 17173-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 17173-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17173-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 17173-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17173-1-AP

  IP result of anti-BPGM (IP:17173-1-AP, 4ug; Detection:17173-1-AP 1:700) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human liver cancer using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293T cells, human plasma, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells, K-562 cells, LNCaP cells, U-87 MG cells, mouse heart tissue, mouse lung tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

17173-1-AP targets BPGM in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BPGM fusion protein Ag10731
• Full Name: 2,3-bisphosphoglycerate mutase
• Calculated Molecular Weight: 259 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC017050
• Gene Symbol: BPGM
• Gene ID (NCBI): 669
• RRID: AB_2274991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IP	Cell Metab; Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis.; Authors - Ping Xu
rat	WB,IF	Brain Res Bull; Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2; Authors - Guoji E
rat	WB	Front Physiol; Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia; Authors - Song Yu