HEK-293 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18017-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 18017-1-AP
IP result of anti-BOLA1 (IP:18017-1-AP, 4ug; Detection:18017-1-AP 1:800) with HepG2 cells lysate 3480ug.
Immunohistochemical analysis of paraffin-embedded human spleen using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 18017-1-AP
Immunofluorescent analysis of HepG2 cells using 18017-1-AP (BOLA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HepG2 cells using 18017-1-AP (BOLA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human lung tissue, human heart tissue, human kidney tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18017-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lung using 18017-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 18017-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 18017-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 18017-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 18017-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 18017-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 18017-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 18017-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of HepG2 using 18017-1-AP
IP result of anti-BOLA1 (IP:18017-1-AP, 4ug; Detection:18017-1-AP 1:800) with HepG2 cells lysate 3480ug.
IF/ICC Figures
IF Staining of HepG2 using 18017-1-AP
Immunofluorescent analysis of HepG2 cells using 18017-1-AP (BOLA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
BOLA1 Polyclonal antibody
Catalog Number
18017-1-AP
Citations
2
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
