BOLA1 Polyclonal antibody

BOLA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18017-1-AP

Synonyms

bolA homolog 1 (E. coli), BolA like protein 1, BOLA1, CGI 143, hBolA, RP11 196G18.18



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung tissue, human heart tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

18017-1-AP targets BOLA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BOLA1 fusion protein Ag12565
Full Name bolA homolog 1 (E. coli)
Calculated Molecular Weight 137 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC063405
Gene Symbol BOLA1
Gene ID (NCBI) 51027
RRIDAB_2066933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BOLA1 antibody 18017-1-APDownload protocol
IHC protocol for BOLA1 antibody 18017-1-APDownload protocol
IF protocol for BOLA1 antibody 18017-1-APDownload protocol
IP protocol for BOLA1 antibody 18017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Res Int

Prognostic Values of BolA Family Member Expression in Hepatocellular Carcinoma

Authors - Dong Wang
mouseWB

bioRxiv

Proteome profiling identifies a link between the mitochondrial pathways and host-microbial sensor ELMO1 following Salmonella infection

Authors - Sajan C Achi