BNIP3L Monoclonal antibody

BNIP3L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1F6A3

Cat no : 68118-1-Ig

Synonyms

BNIP3a, BNIP3H, BNIP3L, NIP3 like protein X, NIP3L, NIX

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Validation Data Gallery

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  WB analysis using 68118-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68118-1-Ig (BNIP3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IHC staining of human prostate cancer using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 68118-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BNIP3L antibody (68118-1-Ig, Clone: 1F6A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, Raji cells
• Positive IHC detected in: rat kidney tissue, human prostate cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68118-1-Ig targets BNIP3L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BNIP3L fusion protein Ag31547
• Full Name: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 35 kD
• GenBank Accession Number: BC001559
• Gene Symbol: BNIP3L
• Gene ID (NCBI): 665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Autophagy; Bunyavirus SFTSV nucleoprotein exploits tufm-mediated mitophagy to impair antiviral innate immunity; Authors - Wen-Kang Zhang