Various lysates were subjected to SDS PAGE followed by western blot with 68118-1-Ig (BNIP3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 68118-1-Ig (BNIP3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
IHC staining of human prostate cancer using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 68118-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BNIP3L antibody (68118-1-Ig, Clone: 1F6A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BNIP3L antibody (68118-1-Ig, Clone: 1F6A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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rat kidney tissue, human prostate cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
BNIP3L/Nix is a mitochondrial protein from the outer membrane that belongs to the BH3-only protein from the BCL2 family. BNIP3L was initially recognized as a proapoptotic protein with milder efficacy in inducing apoptosis compared to other proteins in this family. These phenotypes raised concerns about the molecular function of BNIP3L besides inducing apoptosis. Moreover, studies have shown that BNIP3L is a 24-kDa protein, which is predominantly expressed as a 48-kDa dimer. When analyzed by SDS-PAGE, BNIP3 migrates predominantly as a about 60 kDa dimer in addition to the 30 kDa monomer. (PMID: 34930907, PMID: 32286918)
Various lysates were subjected to SDS PAGE followed by western blot with 68118-1-Ig (BNIP3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
IHC Figures
IHC staining of human prostate cancer using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 68118-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 68118-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BNIP3L antibody (68118-1-Ig, Clone: 1F6A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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