BNIP3L Polyclonal antibody

• Featured Product
• KD/KO Validated

BNIP3L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 12986-1-AP

Synonyms

NIP3L, BNIP3H, BNIP3A, BNIP, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A

---

Validation Data Gallery

• 

  WB analysis using 12986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12986-1-AP (BNIP3L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12986-1-AP (BNIP3L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 12986-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12986-1-AP (BNIP3L antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12986-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12986-1-AP (BNIP3L antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Raji cells, mouse kidney tissue, Jurkat cells, rat kidney tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 3 publications below

Product Information

12986-1-AP targets BNIP3L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BNIP3L fusion protein Ag3624
• Full Name: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC001559
• Gene Symbol: BNIP3L
• Gene ID (NCBI): 665
• RRID: AB_2877901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BNIP3L, also named as BNIP3A, BNIP3H, NIX and NIP3L. Bnip3L is one of the BH3-containing pro-apoptotic proteins and that it targets the mitochondria when inducing apoptosis. It may function as a tumor suppressor. BNIP3L has Dimer form with MW ~70 kDa.

Publications

Species	Application	Title
human	WB	Autophagy; Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations.; Authors - Hongyu Li
mouse	WB	Autophagy; Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.; Authors - Jingru Bai ; KO Validated
human	WB	Cell Rep; Proline restores mitochondrial function and reverses aging hallmarks in senescent cells; Authors - Debanik Choudhury
mouse	WB	Cell Rep; Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.; Authors - Tingting Fu
human	IHC	Neuromuscul Disord; Mitophagy in three cases of immune-mediated necrotizing myopathy associated with anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase autoantibodies: ultrastructural and immunohistochemical studies.; Authors - Shiro Matsubara
rat	WB	Int J Mol Sci; In Vivo Metabolic Responses to Different Formulations of Amino Acid Mixtures for the Treatment of Phenylketonuria (PKU).; Authors - Nadia Giarratana