BMPR1A Recombinant antibody, PBS Only

BMPR1A Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230137B7

Cat no : 82928-1-PBS

Synonyms

ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3, Activin receptor like kinase 3

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Validation Data Gallery

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  WB analysis using 82928-1-RR (same clone as 82928-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82928-1-RR (BMPR1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82928-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 82928-1-RR (same clone as 82928-1-PBS)

  IP result of anti-BMPR1A (IP:82928-1-RR, 4ug; Detection:82928-1-RR 1:2000) with HEK-293 cells lysate 1920 ug. This data was developed using the same antibody clone with 82928-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 82928-1-RR (same clone as 82928-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BMPR1A Recombinant antibody (82928-1-RR, Clone:230137B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82928-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82928-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82928-1-RR against Human BMPR1A were performed. The affinity constant is below 1 pM.

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Product Information

82928-1-PBS targets BMPR1A in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BMPR1A fusion protein Ag3410
• Full Name: bone morphogenetic protein receptor, type IA
• Calculated Molecular Weight: 532 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC028383
• Gene Symbol: BMPR1A
• Gene ID (NCBI): 657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BMPR1A (Bone morphogenetic protein receptor type-1A) is also named as ACVRLK3, ALK3 and SKR5. BMPR1A is an essential receptor for BMP signaling (PMID: 26279380). BMPR1A is necessary for chondrogenesis and osteogenesis (PMID: 32764110). BMPR1A is a cell surface marker of human SuSCs (PMID: 33658353).