BMP2 Monoclonal antibody, PBS Only

BMP2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C5C2

Cat no : 66383-1-PBS

Synonyms

BMP 2, BMP2A, BMP-2, BMP-2A, BMP 2A

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Validation Data Gallery

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  WB analysis of pig bone marrow using 66383-1-Ig (same clone as 66383-1-PBS)

  pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66383-1-Ig (same clone as 66383-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66383-1-Ig (same clone as 66383-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Caco-2 using 66383-1-Ig (same clone as 66383-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 66383-1-Ig (same clone as 66383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 66383-1-Ig (same clone as 66383-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

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Product Information

66383-1-PBS targets BMP2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: BMP2 fusion protein Ag13501
• Full Name: bone morphogenetic protein 2
• Calculated Molecular Weight: 396 aa, 44 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC069214
• Gene Symbol: BMP2
• Gene ID (NCBI): 650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. Two band isofroms of BMP2 are present (PMID: 35846837).