NeutraKine® BMP-7 Monoclonal antibody

NeutraKine® BMP-7 Monoclonal Antibody for FC (Intra), Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra), Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

3E9G7

Cat no : 69011-1-Ig

Synonyms

BMP7, Eptotermin alfa, Bone morphogenetic protein 7, BMP-7, BMP 7

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Validation Data Gallery

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  Neutralization experiment of NeutraKine® BMP-7 using 69011-1-Ig

  Recombinant human BMP-7 (Cat.NO. HZ-1229) induces alkaline phosphatase production in the ATDC-5 cell line (using pNPP as chromogenic substrate for detection) in a dose dependent manner (blue curve, bottom X-left Y). The activity of human BMP-7 (200 ng/mL HZ-1229) is neutralized by mouse anti-human BMP-7 antibody 69011-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-2 μg/mL.

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  FC experiment of HEK-293 using 69011-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human NeutraKine® BMP-7 (69011-1-Ig, Clone:3E9G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Neutralization

Product Information

69011-1-Ig targets NeutraKine® BMP-7 in FC (Intra), Neutralization, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine BMP-7 protein HZ-1229
• Full Name: bone morphogenetic protein 7
• Gene Symbol: BMP7
• Gene ID (NCBI): 655
• RRID: AB_2882929
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

This antibody can be used to neutralize the bioactivity of BMP-7.