BLZF1 Polyclonal antibody, PBS Only

BLZF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16354-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of K-562 using 16354-1-AP (same clone as 16354-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 16354-1-AP (same clone as 16354-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 16354-1-AP (same clone as 16354-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16354-1-AP (same clone as 16354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16354-1-AP (same clone as 16354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 16354-1-AP (same clone as 16354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 16354-1-AP (same clone as 16354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

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Product Information

16354-1-PBS targets BLZF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BLZF1 fusion protein Ag9564
• Full Name: basic leucine zipper nuclear factor 1
• Calculated Molecular Weight: 400 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC020716
• Gene Symbol: BLZF1
• Gene ID (NCBI): 8548
• RRID: AB_2918045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BLZF1 (basic leucine zipper nuclear factor 1), also known as JEM1 or Golgin-45, maps to human chromosome 1q24, encodes for a 3 kb mRNA that is translated into a 45 kDa protein. BLZF1 is a member of b-ZIP family, and has been implicated in epigenetic regulation and Wnt/β-catenin signaling.