BLM Polyclonal antibody

BLM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30254-1-AP

Synonyms

BLM, Bloom syndrome protein, BS, DNA helicase, RecQ like type 2, RecQ protein like 3, RECQ2, RECQL2, RECQL3

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Validation Data Gallery

• 

  WB analysis using 30254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HEK-293T cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30254-1-AP targets BLM in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BLM fusion protein Ag32996
• Full Name: Bloom syndrome, RecQ helicase-like
• Calculated Molecular Weight: 159 kDa
• Observed Molecular Weight: 150-159 kDa
• Gene Symbol: BLM
• Gene ID (NCBI): 641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BLM participates in DNA replication and repair, it is involved in 5'-end resection of DNA during double-strand break (DSB) repair. BLM can also be recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; YY1 is involved in homologous recombination inhibition at guanine quadruplex sites in human cells; Authors - Xinyu Cui