BIVM Polyclonal antibody

BIVM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25432-1-AP

Synonyms

BIVM

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Validation Data Gallery

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  WB analysis of Raji using 25432-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25432-1-AP (BIVM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 25432-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25432-1-AP (BIVM Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 25432-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25432-1-AP (BIVM Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IHC detected in: human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25432-1-AP is BIVM Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BIVM fusion protein Ag22087
• Full Name: basic, immunoglobulin-like variable motif containing
• Calculated Molecular Weight: 503 aa, 57 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC075084
• Gene Symbol: BIVM
• Gene ID (NCBI): 54841
• RRID: AB_2880076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIVM has been identified using an electronic search based on the conservation of short sequence motifs within the variable region of immunoglobulin (Ig) genes. It is tightly linked (41 bp) and in the opposite transcriptional orientation to MGC5302 (also known as KDEL1 and EP58) in human. BIVM has two isoforms with MW 57 kDa and 32 kDa.