Tested Applications
Positive WB detected in | DU 145 cells, LNCaP cells, PC-3 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IHC | See 1 publications below |
Product Information
29760-1-AP targets BIRC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | BIRC6 fusion protein Ag30729 Predict reactive species |
Full Name | baculoviral IAP repeat-containing 6 |
Calculated Molecular Weight | 530 kDa |
Observed Molecular Weight | 530 kDa |
GenBank Accession Number | NM_016252 |
Gene Symbol | BIRC6 |
Gene ID (NCBI) | 57448 |
RRID | AB_2923604 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NR09 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BIRC6 (Baculoviral IAP Repeat Containing 6), also known as BRUCE or APOLLON, is a member of the Inhibitors of Apoptosis Protein (IAP) family with a single BIR domain at its N-terminal and an active ubiquitin-conjugating (UBC) enzyme domain at its C-terminal(PMID: 23409057). In addition to its IAP activity, BIRC6 has the distinctive property of activity as a chimeric E2/E3 ubiquitin ligase in mammals. With its UBC domain, BIRC6 promotes the degradation of Smac and inhibits the activity of caspase-9, which play key roles in the initiation of apoptosis(PMID: 25933218). BIRC6 is highly expressed in many cancer types, where elevated expression of BIRC6 is significantly correlated with poor clinical outcome(PMID: 34729249).
Protocols
Product Specific Protocols | |
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WB protocol for BIRC6 antibody 29760-1-AP | Download protocol |
IHC protocol for BIRC6 antibody 29760-1-AP | Download protocol |
IF protocol for BIRC6 antibody 29760-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Genet Identification of an autophagy-related 12-lncRNA signature and evaluation of NFYC-AS1 as a pro-cancer factor in lung adenocarcinoma |