BIRC6 Polyclonal antibody

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  WB analysis using 29760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29760-1-AP (BIRC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 29760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29760-1-AP (BIRC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 29760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29760-1-AP (BIRC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 29760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BIRC6 antibody (29760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

BIRC6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 29760-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

APOLLON, BIRC6, BRUCE, FLJ13726, FLJ13786, KIAA1289

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Tested Applications

• Positive WB detected in: DU 145 cells, LNCaP cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

29760-1-AP targets BIRC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BIRC6 fusion protein Ag30729
• Full Name: baculoviral IAP repeat-containing 6
• Calculated Molecular Weight: 530 kDa
• Observed Molecular Weight: 530 kDa
• GenBank Accession Number: NM_016252
• Gene Symbol: BIRC6
• Gene ID (NCBI): 57448
• RRID: AB_2923604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NR09
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIRC6 (Baculoviral IAP Repeat Containing 6), also known as BRUCE or APOLLON, is a member of the Inhibitors of Apoptosis Protein (IAP) family with a single BIR domain at its N-terminal and an active ubiquitin-conjugating (UBC) enzyme domain at its C-terminal(PMID: 23409057). In addition to its IAP activity, BIRC6 has the distinctive property of activity as a chimeric E2/E3 ubiquitin ligase in mammals. With its UBC domain, BIRC6 promotes the degradation of Smac and inhibits the activity of caspase-9, which play key roles in the initiation of apoptosis(PMID: 25933218). BIRC6 is highly expressed in many cancer types, where elevated expression of BIRC6 is significantly correlated with poor clinical outcome(PMID: 34729249).

Publications

Species	Application	Title
mouse,human	WB,IHC	Front Genet; Identification of an autophagy-related 12-lncRNA signature and evaluation of NFYC-AS1 as a pro-cancer factor in lung adenocarcinoma; Authors - Fang Tong