BIN3 Polyclonal antibody, PBS Only

BIN3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11215-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of K-562 using 11215-1-AP (same clone as 11215-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11215-1-AP (same clone as 11215-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 11215-1-AP (same clone as 11215-1-PBS)

  IP result of anti-BIN3 (IP:11215-1-AP, 4ug; Detection:11215-1-AP 1:300) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11215-1-AP (same clone as 11215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11215-1-AP (BIN3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

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Product Information

11215-1-PBS targets BIN3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BIN3 fusion protein Ag1713
• Full Name: bridging integrator 3
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC009824
• Gene Symbol: BIN3
• Gene ID (NCBI): 55909
• RRID: AB_2063649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Bridging integrator 3 (BIN3) is a conserved BAR (Bin/amphiphysin/ Rvs) domain adaptor protein (PMID: 11274158). BAR domain proteins, sharing an N-terminal BAR domain, have been implicated in diverse cellular processes, including endocytosis, vesicle fusion, cell polarity, actin organization, signal transduction, transcription repression, and tumor suppression (PMID: 16524918). BIN3 is involved in cytokinesis and septation where it has a role in the localization of F-actin (PMID: 11274158).