BIN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

BIN2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14245-1-AP

Synonyms

BIN2, BRAP 1, BRAP1, bridging integrator 2

---

Validation Data Gallery

• 

  WB analysis using 14245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 14245-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14245-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 14245-1-AP

  IP result of anti-BIN2 (IP:14245-1-AP, 4ug; Detection:14245-1-AP 1:400) with Jurkat cells lysate 2000ug.

• 

  IHC staining of human spleen using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse heart using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14245-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: Jurkat cells, U-937 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human spleen tissue, mouse heart tissue, human heart tissue, human skin tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

14245-1-AP targets BIN2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BIN2 fusion protein Ag5502
• Full Name: bridging integrator 2
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC047686
• Gene Symbol: BIN2
• Gene ID (NCBI): 51411
• RRID: AB_2063647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)

Publications

Species	Application	Title
mouse	WB,IP	J Clin Invest; BIN2 orchestrates platelet calcium signaling in thrombosis and thrombo-inflammation.; Authors - Julia Volz ; KO Validated

Related Products

BIN1 Polyclonal antibody

14647-1-AP

ARHGEF7 Polyclonal antibody

14092-1-AP

Endophilin 1 Polyclonal antibody

12435-1-AP