BIK Monoclonal antibody

BIK Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B1C10

Cat no : 68438-1-Ig

Synonyms

Apoptosis inducer NBK, BIK, BIP1, BP4, NBK

---

Validation Data Gallery

• 

  WB analysis using 68438-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68438-1-Ig (BIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: SW480 cells, Caco-2 cells, THP-1 cells, Raji cells, Daudi cells, Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68438-1-Ig targets BIK in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: BIK fusion protein Ag6353
• Full Name: BCL2-interacting killer (apoptosis-inducing)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC001599
• Gene Symbol: BIK
• Gene ID (NCBI): 638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.