TGFBI / BIGH3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TGFBI / BIGH3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Neutralization, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

68

Reviews (1)

Cat no : 10188-1-AP

Synonyms

TGFBI, RGD-containing collagen-associated protein, RGD-CAP, Kerato-epithelin, BIGH3

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Validation Data Gallery

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  WB analysis using 10188-1-AP

  Mouse eye tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10188-1-AP

  WB result of TGFBI / BIGH3 antibody (10188-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBI / BIGH3 transfected HeLa cells.

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  WB analysis of mouse eye using 10188-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10188-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 10188-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 10188-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10188-1-AP

  IP result of anti-TGFBI / BIGH3 (IP:10188-1-AP, 3ug; Detection:10188-1-AP 1:300) with HeLa cells lysate 1000ug.

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  IHC staining of human kidney using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse eye using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 10188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 10188-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells, untreated (left) or TGF-β-treated (right), using TGFBI / BIGH3 antibody (10188-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Y79 using 10188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using TGFBI / BIGH3 antibody (10188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Y79 using 10188-1-AP

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug Anti-Human TGFBI / BIGH3 (10188-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse eye tissue, mouse liver tissue, human kidney tissue, Y79 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human liver cancer tissue, mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: TGF beta 1 treated A549 cells, Y79 cells
• Positive FC (Intra) detected in: Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 41 publications below
• IHC: See 34 publications below
• IF: See 14 publications below
• IP: See 4 publications below
• FC: See 1 publications below

Product Information

10188-1-AP targets TGFBI / BIGH3 in WB, IHC, IF/ICC, FC (Intra), IP, Neutralization, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGFBI / BIGH3 fusion protein Ag0241
• Full Name: transforming growth factor, beta-induced, 68kDa
• Calculated Molecular Weight: 683 aa, 75 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC000097
• Gene Symbol: TGFBI
• Gene ID (NCBI): 7045
• RRID: AB_2202311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Publications

Species	Application	Title
human	WB, IF	Nat Cell Biol; Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets.; Authors - Qin Zhang
human	WB	Nat Protoc; Comprehensive isolation of extracellular vesicles and nanoparticles; Authors - Qin Zhang
human	IP	Sci Adv; Acetylated K676 TGFBIp as a severity diagnostic blood biomarker for SARS-CoV-2 pneumonia.; Authors - Hee Ho Park
mouse	WB,IHC	Genes Dev; Extracellular matrix protein betaig-h3/TGFBI promotes metastasis of colon cancer by enhancing cell extravasation.; Authors - Ma Chaoyu C ; KD Validated
human	WB,IP	Biomaterials; Dual peptide-dendrimer conjugate inhibits acetylation of transforming growth factor β-induced protein and improves survival in sepsis.; Authors - Wonhwa Lee
human	WB,IHC,IF	Theranostics; TGFBI secreted by tumor-associated macrophages promotes glioblastoma stem cell-driven tumor growth via integrin αvβ5-Src-Stat3 signaling.; Authors - Peng Peng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carmelo (Verified Customer) (04-25-2022)

I tested the abs 1:1000 ON at 4C on 40ug spinal cord protein lysates. unfortunately I was not able to distinguish a proper band at the expected size. I will run further optimisation in the incoming weeks.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Spinal Cord

TGFBI / BIGH3 Antibody Western Blot validation (1:1000 dilution) in Mouse Spinal Cord (Cat no:10188-1-AP)

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