BHLHE40 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

BHLHE40 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17895-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 17895-1-AP (same clone as 17895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17895-1-AP (BHLHE40 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 17895-1-AP (same clone as 17895-1-PBS)

  IP result of anti-BHLHE40 (IP:17895-1-AP, 4ug; Detection:17895-1-AP 1:1000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 17895-1-AP (same clone as 17895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 17895-1-AP (same clone as 17895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 17895-1-AP (same clone as 17895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 17895-1-AP (same clone as 17895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 17895-1-AP (same clone as 17895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 17895-1-AP (same clone as 17895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

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Product Information

17895-1-PBS targets BHLHE40 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BHLHE40 fusion protein Ag12236
• Full Name: basic helix-loop-helix family, member e40
• Calculated Molecular Weight: 412 aa, 46 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC082238
• Gene Symbol: BHLHE40
• Gene ID (NCBI): 8553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BHLHE40 (Basic Helix-Loop-Helix Family Member E40), also known as BHLHB2, STRA13, DEC1, or SHARP2, is a member of the basic helix-loop-helix (bHLH) protein family, a large superfamily of transcriptional regulators expressed in many organisms. BHLHE40 is known to regulate a wide variety of essential cellular processes, including cell cycle, cellular proliferation, programmed cell death, cellular development and differentiation, as well as circadian rhythms (PMID: 34551158). It is reported that BHLHE40 is overexpressed in gastric, breast, and brain tumors; and downregulated in colorectal, esophageal, pancreatic and lung cancer (PMID: 32577154).