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Bestrophin-1 Monoclonal antibody

Bestrophin-1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4D5F1

Cat no : 60326-1-Ig

Synonyms

ARB, BEST, BEST1, bestrophin 1, Bestrophin-1, BMD, TU15B, VMD2

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using Bestrophin-1 Monoclonal antibody (60326-1-Ig)

    WB analysis of Y79 using 60326-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 60326-1-Ig (Bestrophin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using Bestrophin-1 Monoclonal antibody (60326-1-Ig)

    WB analysis of Transfected HEK-293 using 60326-1-Ig

    WB result of Bestrophin-1 antibody (60326-1-Ig; 1:1000; room temperature for 1.5 hours) with negatove control and Bestrophin-1 overexpressed Transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of Y79 cells using Bestrophin-1 Monoclonal antibody (60326-1-Ig)

    IF Staining of Y79 using 60326-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Bestrophin-1 antibody (60326-1-Ig, Clone: 4D5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inY79 cells, Transfected HEK-293 cells
Positive IF/ICC detected inY79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

60326-1-Ig targets Bestrophin-1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Bestrophin-1 fusion protein Ag15129
Full Name bestrophin 1
Calculated Molecular Weight 585 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC015220
Gene Symbol Bestrophin/BEST1
Gene ID (NCBI) 7439
RRIDAB_2881436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bestrophin-1 (BEST1) is a 68 kDa transmembrane protein which belongs to the bestrophin family of anion channels. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. Studies show that bestrophin 1 functions as a Ca(2+)-dependent Cl(-) channel and modulator of voltage-dependent L-type Ca(2+) channels. It may also contribute to the basolateral cell conductance in airway epithelial cells. This protein is encoded by the VMD2 gene. Mutations in VMD2 can lead to different types of retinal or macular degenerations, including Best vitelliform macular dystrophy (BMD), autosomal recessive bestrophinopathy (ARB), autosomal dominant vitreoretinochoroidopathy (ADVIRC) and adult-onset vitelliform macular dystrophy (AVMD).

Protocols

Product Specific Protocols
WB protocol for Bestrophin-1 antibody 60326-1-IgDownload protocol
IF protocol for Bestrophin-1 antibody 60326-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu
View Article