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BEND3 Polyclonal antibody, PBS Only

BEND3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23101-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BEND3 Polyclonal antibody (23101-1-AP)

    WB analysis using 23101-1-AP (same clone as 23101-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23101-1-AP (BEND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BEND3 Polyclonal antibody (23101-1-AP)

    IHC staining of human liver cancer using 23101-1-AP (same clone as 23101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23101-1-AP (BEND3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BEND3 Polyclonal antibody (23101-1-AP)

    IHC staining of human liver cancer using 23101-1-AP (same clone as 23101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23101-1-AP (BEND3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BEND3 Polyclonal antibody (23101-1-AP)

    IF Staining of HEK-293 using 23101-1-AP (same clone as 23101-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

Product Information

23101-1-PBS targets BEND3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BEND3 fusion protein Ag19137
Full Name BEN domain containing 3
Calculated Molecular Weight 828 aa, 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC131628
Gene Symbol BEND3
Gene ID (NCBI) 57673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)