BEGAIN Polyclonal antibody

BEGAIN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14956-1-AP

Synonyms

BEGAIN, KIAA1446

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Validation Data Gallery

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  WB analysis using 14956-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14956-1-AP (BEGAIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 14956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14956-1-AP (BEGAIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, JAR cells, SH-SY5Y cells
• Positive IHC detected in: human pancreas cancer tissue, mouse cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14956-1-AP targets BEGAIN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BEGAIN fusion protein Ag6794
• Full Name: brain-enriched guanylate kinase-associated homolog (rat)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC002607
• Gene Symbol: BEGAIN
• Gene ID (NCBI): 57596
• RRID: AB_2064487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	PLoS One; The First Nationwide Survey and Genetic Analyses of Bardet-Biedl Syndrome in Japan.; Authors - Makito Hirano