BCL9 Recombinant antibody, PBS Only (Capture)

BCL9 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240682A9

Cat no : 83760-3-PBS

Synonyms

Bcl-9, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein, B cell CLL/lymphoma 9

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Validation Data Gallery

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  IF Staining of HeLa using 83760-3-RR (same clone as 83760-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL9 antibody (83760-3-RR, Clone: 240682A9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83760-3-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 83760-3-RR (same clone as 83760-3-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Bcl9 Recombinant Antibody (83760-3-RR, Clone:240682A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83760-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00739-2

  Cytometric bead array standard curve of MP00739-2, BCL9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83760-3-PBS. Detection antibody: 83760-5-PBS. Standard: Ag19158. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00739-3

  Cytometric bead array standard curve of MP00739-3, BCL9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83760-3-PBS. Detection antibody: 83760-4-PBS. Standard: Ag19158. Range: 0.625-40 ng/mL.

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Product Information

83760-3-PBS targets BCL9 as part of a matched antibody pair:

MP00739-2: 83760-3-PBS capture and 83760-5-PBS detection (validated in Cytometric bead array)

MP00739-3: 83760-3-PBS capture and 83760-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCL9 fusion protein Ag19158
• Full Name: B-cell CLL/lymphoma 9
• Calculated Molecular Weight: 1426 aa, 149 kDa
• GenBank Accession Number: BC116451
• Gene Symbol: BCL9
• Gene ID (NCBI): 607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.