BCL2L2 Polyclonal antibody

BCL2L2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 16026-1-AP

Synonyms

Apoptosis regulator Bcl W, BCL2L2, KIAA0271

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Validation Data Gallery

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  WB analysis of HL-60 using 16026-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16026-1-AP (BCL2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16026-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16026-1-AP (BCL2L2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16026-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16026-1-AP (BCL2L2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 1 publications below

Product Information

16026-1-AP targets BCL2L2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL2L2 fusion protein Ag8863
• Full Name: BCL2-like 2
• Calculated Molecular Weight: 193 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC021198
• Gene Symbol: BCL2L2
• Gene ID (NCBI): 599
• RRID: AB_2827646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Peptides Isolated from Yak Milk Residue Exert Antioxidant Effects through Nrf2 Signal Pathway.; Authors - Feiyan Yang
human	WB	Cancers (Basel); Gemcitabine Cooperates with Everolimus to Inhibit the Growth of and Sensitize Malignant Meningioma Cells to Apoptosis Induced by Navitoclax, an Inhibitor of Anti-Apoptotic BCL-2 Family Proteins.; Authors - Masahiro Yamamoto
human	WB	iScience; HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6.; Authors - Menghui Yin
human	WB	PLoS One; Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli.; Authors - Yu Guohua G
rat	WB	Eur J Pharmacol; GSK-3β-mediated activation of NLRP3 inflammasome leads to pyroptosis and apoptosis of rat cardiomyocytes and fibroblasts.; Authors - Shu-Hui Wang
human	WB	Tumour Biol; MicroRNA-15a induces cell apoptosis and inhibits metastasis by targeting BCL2L2 in non-small cell lung cancer.; Authors - Tian Yang