BCL2L12 Polyclonal antibody

BCL2L12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21969-1-AP

Synonyms

BCL2L12, BPR

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Validation Data Gallery

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  WB analysis of PC-3 using 21969-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21969-1-AP (BCL2L12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 21969-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21969-1-AP (BCL2L12 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 21969-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21969-1-AP (BCL2L12 Antibody) at dilution of 1:50.

• 

  IF Staining of PC-3 using 21969-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 21969-1-AP (BCL2L12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21969-1-AP targets BCL2L12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL2L12 fusion protein Ag16856
• Full Name: BCL2-like 12 (proline rich)
• Calculated Molecular Weight: 334 aa, 37 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC104005
• Gene Symbol: BCL2L12
• Gene ID (NCBI): 83596
• RRID: AB_2878956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.