BCL2 Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

BCL2 Monoclonal Antibody for IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H8C6

Cat no : 60178-1-PBS

Synonyms

BCL-2, 4H8C6

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Validation Data Gallery

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  IP experiment of U-937 using 60178-1-Ig (same clone as 60178-1-PBS)

  IP result of anti-BCL2 (IP:60178-1-Ig, 5ug; Detection:60178-1-Ig 1:1000) with U-937 cells lysate 4000ug. This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:1500 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60178-1-Ig (same clone as 60178-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50290-2

  Cytometric bead array standard curve of MP50290-2, BCL2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60178-1-PBS. Detection antibody: 60178-2-PBS. Standard:Ag3508. Range: 1.563-100 ng/mL.

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Product Information

60178-1-PBS targets BCL2 as part of a matched antibody pair:

MP50290-2: 60178-1-PBS capture and 60178-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCL2 fusion protein Ag3508
• Full Name: B-cell CLL/lymphoma 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC027258
• Gene Symbol: BCL2
• Gene ID (NCBI): 596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

1) What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.