BCL10 Polyclonal antibody

• Featured Product
• KD/KO Validated

BCL10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17732-1-AP

Synonyms

CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10

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Validation Data Gallery

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  WB analysis using 17732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17732-1-AP (BCL10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17732-1-AP

  WB result of BCL10 antibody (17732-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL10 transfected K-562 cells.

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  IHC staining of human breast cancer using 17732-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17732-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 17732-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 17732-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17732-1-AP (BCL10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17732-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCL10 antibody (17732-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17732-1-AP targets BCL10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL10 fusion protein Ag12170
• Full Name: B-cell CLL/lymphoma 10
• Calculated Molecular Weight: 233 aa, 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC053617
• Gene Symbol: BCL10
• Gene ID (NCBI): 8915
• RRID: AB_2064860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.