BCKDHA Polyclonal antibody

• Featured Product
• KD/KO Validated

BCKDHA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 30028-1-AP

Synonyms

BCKDHA, FLJ45695, MSU, MSUD1, OVD1A

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Validation Data Gallery

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  WB analysis using 30028-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30028-1-AP (BCKDHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 30028-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 30028-1-AP (BCKDHA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 30028-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30028-1-AP (BCKDHA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 30028-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30028-1-AP (BCKDHA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, rat liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

30028-1-AP targets BCKDHA in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCKDHA fusion protein Ag32613
• Full Name: branched chain keto acid dehydrogenase E1, alpha polypeptide
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 42-50 kDa
• GenBank Accession Number: BC008933
• Gene Symbol: BCKDHA
• Gene ID (NCBI): 593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), the gene encoding the regulated subunit of BCKDC was only one of two primary susceptibility genes identified that affected the risk of both type 2 diabetes mellitus (T2DM) and obesity (PMID: 25287287). BIX01294 transcriptionally downregulated the transcription of BCKDHA, which is essential for fueling the tricarboxylic acid (TCA) cycle. Studies have shown that KDM3A, a Jumonji histone demethylase, epigenetically regulates BCKDHA expression by binding to the BCKDHA gene promoter (PMID: 34876693). Moreover, at least four genes including BCKDHA, branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), dihydrolipoamide dehydrogenase (DLD), and dihydrolipoamide branched chain transacylase E2 (DBT) have been reported to be the causative gene for Maple syrup urine disease (MSUD) (PMID: 34187135).

Publications

Species	Application	Title
rat	WB	Clin Nutr; The effects of vitamin B6 on the nutritional support of BCAAs-enriched amino acids formula in rats with partial gastrectomy; Authors - Ying Wang
mouse	WB	J Thromb Haemost; Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling; Authors - Haojie Jiang ; KO Validated