BCAT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

BCAT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

22

Reviews (2)

Cat no : 16417-1-AP

Synonyms

PP18, Placental protein 18, ECA40, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, mitochondrial

---

Validation Data Gallery

• 

  WB analysis using 16417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16417-1-AP (BCAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16417-1-AP

  WB result of BCAT2 antibody (16417-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAT2 transfected MCF-7 cells.

• 

  WB analysis of L02 using 16417-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16417-1-AP (BCAT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 16417-1-AP (BCAT2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 16417-1-AP (BCAT2 Antibody) at dilution of 1:50.

• 

  IF Staining of C2C12 using 16417-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16417-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human BCAT2 (16417-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, L02 cells, Jurkat cells, Raji cells
• Positive IHC detected in: human skin tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

16417-1-AP targets BCAT2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCAT2 fusion protein Ag9573
• Full Name: branched chain aminotransferase 2, mitochondrial
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC001900
• Gene Symbol: BCAT2
• Gene ID (NCBI): 587
• RRID: AB_10792411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).

Publications

Species	Application	Title
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
human,mouse	WB	Nat Commun; Glucose promotes cell growth by suppressing branched-chain amino acid degradation.; Authors - Dan Shao
human	WB, IHC	Basic Res Cardiol; Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.; Authors - Qiong Lai
human	WB	Cancer Res; Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.; Authors - Bo Zhang
mouse	WB	Diabetes; Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.; Authors - Na Tian
human	WB	Cell Mol Life Sci; Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.; Authors - Bo Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kamal (Verified Customer) (02-15-2024)

Antibody works well when diluted in 1X TBST. Bands appeared at 40 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue

FH

chao (Verified Customer) (01-30-2020)

Strong, clear band for heart lysates.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart