BCAT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BCAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

29

Reviews (2)

Cat no : 13640-1-AP

Synonyms

PNAS 121, MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic

---

Validation Data Gallery

• 

  WB analysis of HeLa using 13640-1-AP

  WB result of BCAT1/ECA39 antibody (13640-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAT1/ECA39 transfected HeLa cells.

• 

  WB analysis using 13640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13640-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13640-1-AP (BCAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13640-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCAT1 antibody (13640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, Neuro-2a cells
• Positive IHC detected in: mouse brain tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 25 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

13640-1-AP targets BCAT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCAT1 fusion protein Ag4574
• Full Name: branched chain aminotransferase 1, cytosolic
• Calculated Molecular Weight: 320 aa, 36 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC033864
• Gene Symbol: BCAT1
• Gene ID (NCBI): 586
• RRID: AB_2063569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCAT1, also named branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine, and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149). The 13640-1-AP antibody detects the bands around 43-45 kDa for different isoforms and higher molecular weight for phosphorylation modification of this protein.

Publications

Species	Application	Title
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian ; KO Validated
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
human	WB, IHC	Basic Res Cardiol; Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.; Authors - Qiong Lai
human	WB	Cancer Res; Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.; Authors - Bo Zhang
human	WB	Cell Mol Life Sci; Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.; Authors - Bo Zhang ; KO Validated
human	WB,IHC	Oncotarget; BCAT1 expression associates with ovarian cancer progression: possible implications in altered disease metabolism.; Authors - Zhi-Qiang Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kis (Verified Customer) (12-08-2022)

This antibody was used for (1:1000) WB and (1:500) for IHC. We got satisfactory results with this ab.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

BCAT1 Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Human Liver cells (Cat no:13640-1-AP)

FH

Iru (Verified Customer) (06-18-2020)

It works fine with FTE cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells