BAP29 Polyclonal antibody

BAP29 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 15796-1-AP

Synonyms

BCR-associated protein 29, B-cell receptor-associated protein 29, BCAP29

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Validation Data Gallery

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  WB analysis using 15796-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 15796-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 15796-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lymphoma using 15796-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HeLa using 15796-1-AP

  Immunofluorescent analysis of Hela cells, using BCAP29 antibody 15796-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HEK-293 using 15796-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug BAP29 Polyclonal antibody (15796-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, COLO 320 cells, Raji cells, U-87 MG cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15796-1-AP targets BAP29 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAP29 fusion protein Ag8505
• Full Name: B-cell receptor-associated protein 29
• Calculated Molecular Weight: 241 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC008478
• Gene Symbol: BAP29
• Gene ID (NCBI): 55973
• RRID: AB_2065118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xin (Verified Customer) (03-19-2021)

The protein ran slightly lower than the predicted size (28kDa), it was detected somewhere around 24kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cells

BAP29 Antibody Western Blot validation (1:1000 dilution) in A549 cells (Cat no:15796-1-AP)