BCAM Polyclonal antibody

BCAM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26528-1-AP

Synonyms

AU, Auberger B antigen, Basal cell adhesion molecule, BCAM, CD239, F8/G253 antigen, LU, Lutheran antigen, MSK19

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Validation Data Gallery

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  WB analysis using 26528-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26528-1-AP (BCAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, HT-29 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26528-1-AP targets BCAM in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCAM fusion protein Ag24753
• Full Name: basal cell adhesion molecule (Lutheran blood group)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 78 kDa, 85 kDa
• GenBank Accession Number: BC050450
• Gene Symbol: BCAM
• Gene ID (NCBI): 4059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Basal cell adhesion molecule (Lu/BCAM) is a 90 kDa membrane-bound glycoprotein of the immunoglobulin superfamily (IgSF), functioning as a receptor for the extracellular matrix protein, laminin. BCAM, involved in cell adhesion and migration, can also promote tumor metastasis and has been elucidated to play a functional role in the metastasis of thyroid cancer and gastric cancer (PMID: 35941663). Two Lu/BCAM glycoprotein (gp) isoforms are present on human RBCs, Lu (85 kDa) and Lu(v13) (78 kDa); they result from the alternative splicing of intron 13 and differ by the lengths of their cytoplasmic domains (PMID: 23160466)..