BBS7 Polyclonal antibody
BBS7 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 18961-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human brain tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human testis tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 8 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
IP | See 2 publications below |
Product Information
18961-1-AP targets BBS7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | BBS7 fusion protein Ag4601 |
Full Name | Bardet-Biedl syndrome 7 |
Calculated Molecular Weight | 715 aa, 80 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | BC032691 |
Gene Symbol | BBS7 |
Gene ID (NCBI) | 55212 |
RRID | AB_2064998 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BBS7, also named as BBS2L1, is a component of the BBSome complex which is required for ciliogenesis but is dispensable for centriolar satellite function. BBS7 is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome.
Protocols
Product Specific Protocols | |
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WB protocol for BBS7 antibody 18961-1-AP | Download protocol |
IHC protocol for BBS7 antibody 18961-1-AP | Download protocol |
IP protocol for BBS7 antibody 18961-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. | ||
PLoS Genet A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened. | ||
PLoS Genet The Centriolar Satellite Protein AZI1 Interacts with BBS4 and Regulates Ciliary Trafficking of the BBSome. | ||
PLoS Genet BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment. | ||
Hum Mol Genet Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome. | ||
FASEB J Rhodopsin mislocalization drives ciliary dysregulation in a novel autosomal dominant retinitis pigmentosa knock-in mouse model |