BBS10 Monoclonal antibody

BBS10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4C6A10

Cat no : 66679-1-Ig

Synonyms

Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560

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Validation Data Gallery

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  WB analysis using 66679-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 66679-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, NCCIT cells, L02 cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66679-1-Ig targets BBS10 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BBS10 fusion protein Ag17960
• Full Name: Bardet-Biedl syndrome 10
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC013795
• Gene Symbol: BBS10
• Gene ID (NCBI): 79738
• RRID: AB_2882033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID: 20080638, 19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).