BBS10 Polyclonal antibody

• Featured Product
• KD/KO Validated

BBS10 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12421-2-AP

Synonyms

C12orf58, Bardet-Biedl syndrome 10 protein, Bardet Biedl syndrome 10

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Validation Data Gallery

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  WB analysis of HepG2 using 12421-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12421-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12421-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 cells using 12421-2-AP

  IF result (nuclear and the base of cilia stain) of anti-BBS10 (12421-2-AP; 1:50) with hTERT-RPE1 cell (PFA fixed) by Dr. Moshe Kim.

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, MCF-7 cells
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

12421-2-AP targets BBS10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS10 fusion protein Ag3073
• Full Name: Bardet-Biedl syndrome 10
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC013795
• Gene Symbol: BBS10
• Gene ID (NCBI): 79738
• RRID: AB_2064962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).

Publications

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; Transient ciliogenesis involving Bardet-Biedl syndrome proteins is a fundamental characteristic of adipogenic differentiation.; Authors - Marion Vincent V ; KD Validated
human,mouse	WB,IF	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S
mouse	WB	Cilia; Comparing the Bbs10 complete knockout phenotype with a specific renal epithelial knockout one highlights the link between renal defects and systemic inactivation in mice.; Authors - Noëlle Cognard ; KO Validated