BBOX1 Polyclonal antibody

• 

  WB analysis of human brain using 16099-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16099-1-AP (BBOX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16099-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16099-1-AP (BBOX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16099-1-AP

  Immunofluorescent analysis of HepG2 cells, using BBOX1 antibody 16099-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

BBOX1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16099-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Gamma-BBH, gamma BBH, G BBH, EC:1.14.11.1, BBOX

---

Tested Applications

• Positive WB detected in: human brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

16099-1-AP targets BBOX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBOX1 fusion protein Ag8964
• Full Name: butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1
• Calculated Molecular Weight: 387 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC011034
• Gene Symbol: BBOX1
• Gene ID (NCBI): 8424
• RRID: AB_2243498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75936
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	EMBO J; CRIP1 suppresses BBOX1-mediated carnitine metabolism to promote stemness in hepatocellular carcinoma; Authors - Jing Wang
human	WB,IHC	Biochim Biophys Acta Mol Cell Res; BBOX1 mediates metabolic reprogramming driven by hypoxia and participates in the malignant progress of high-grade serous ovarian cancer; Authors - Jiazhen Huang ; KD Validated