BBOF1 Polyclonal antibody

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  WB analysis of TT using 24337-1-AP

  TT cells were subjected to SDS PAGE followed by western blot with 24337-1-AP (CCDC176 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 24337-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24337-1-AP (BBOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 24337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BBOF1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24337-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C14orf45, Coiled-coil domain-containing protein 176, CCDC176, Basal body-orientation factor 1

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Tested Applications

• Positive WB detected in: mouse testis tissue, TT cells, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24337-1-AP targets BBOF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBOF1 fusion protein Ag19302
• Full Name: chromosome 14 open reading frame 45
• Calculated Molecular Weight: 529 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC104981
• Gene Symbol: BBOF1
• Gene ID (NCBI): 80127
• RRID: AB_3669433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8ND07
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Basal body orientation factor 1 (BBOF1), also known as CCDC176, contains two coiled-coil domains and is evolutionarily conserved in vertebrates. Recently, Xenopus bbof1 has been characterized as a cilia-associated factor that is induced by the transcription factor foxj1 to mediate the alignment and orientation of cilia in epidermal multi-ciliated cells. BBOF1 is required for sperm flagellar axoneme maintenance and male fertility in mice. BBOF1 expression is elevated during meiosis, and it is accumulated in round spermatids. BBOF1 deletion has compromised effects on growth, survival, and spermatogenesis.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (11-25-2024)

recognized a protein at the centrosome, but quite weak staining compared with background

• Applications: Immunofluorescence